SARS-CoV-2 / gripp A / gripp B
Mahsulot nomi
HWTS-RT148-SARS-CoV-2/influenza A/influenza B nuklein kislotasi kombinatsiyalangan aniqlash to'plami (Fluoresans PCR)
Kanal
| Kanal nomi | PCR-Mix 1 | PCR-Mix 2 |
| FAM kanali | ORF1ab geni | IVA |
| VIC/HEX kanali | Ichki nazorat | Ichki nazorat |
| CY5 kanali | N gen | / |
| ROX kanali | E gen | IVB |
Texnik parametrlar
| Saqlash | -18℃ |
| Yaroqlilik muddati | 12 oy |
| Namuna turi | nazofarengeal tamponlar va orofaringeal tamponlar |
| Maqsad | SARS-CoV-2 uchta maqsad (Orf1ab, N va E genlari)/gripp A/gripp B |
| Ct | ≤38 |
| CV | ≤10,0% |
| LoD | SARS-CoV-2: 300 nusxa/ml A grippi virusi: 500 nusxa/ml gripp B virusi: 500 nusxa/ml |
| O'ziga xoslik | a) Oʻzaro test natijalari shuni koʻrsatdiki, toʻplam inson koronavirusi SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respirator sintsitial virus A va B, parainfluenza virusi 1, 2 va C virusi, A virusi, 1, rhino virusi, A virusi va virusi bilan mos keladi. 2, 3, 4, 5, 7 va 55, inson metapnevmovirusi, A, B, C va D enterovirusi, odamning sitoplazmatik o'pka virusi, EB virusi, qizamiq virusi, odam sitomegalovirusi, rotavirus, norovirus, parotit virusi, varikella zoster virusi, Mycoplasma pneumoniaeion, pneumoniaeiae, Chronovirus Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata yerocine va C o'rtasida reaktsiya yo'q edi. neoformanlar. b) Interferentsiyaga qarshi qobiliyat: tanlangan musin (60mg/ml), 10% (V/V) inson qoni, difenilefrin (2mg/ml), gidroksimetilzolin (2mg/ml), natriy xlorid (tarkibida konservant) (20mg/ml), beklometazon (20mg/ml), beklometazon (20mg/ml), flunizon (20 mkg / ml), triamsinolon asetonid (2 mg / ml), budesonid (2 mg / ml), mometazon (2 mg / ml), flutikazon (2 mg / ml), gistamin gidroxloridi (5 mg / ml), a-Interferon (800 IU / mL), a-Interferon (800 IU / mL) (10mg/ml), oseltamivir (60ng/ml), pramivir (1mg/ml), lopinavir (500mg/ml), ritonavir (60mg/ml), mupirosin (20mg/ml), azitromitsin (1mg/ml), seproten (40mg/ml), seproten (40mg/ml), levofloksatsin (10 mkg/ml) va tobramitsin (0,6 mg/ml). Natijalar shuni ko'rsatdiki, yuqoridagi konsentratsiyalarda aralashuvchi moddalar patogenlarni aniqlash natijalariga hech qanday interferentsiyaga ega emas. |
| Amaldagi asboblar | Amaliy Biosystems 7500 Real-Time PCR tizimlari Amaliy Biosystems 7500 Fast Real-Time PCR tizimlari SLAN ®-96P Real-Time PCR tizimlari QuantStudio™ 5 real vaqtda PCR tizimlari LightCycler®480 Real-Time PCR tizimi LineGene 9600 Plus real vaqtda PCR aniqlash tizimi MA-6000 real vaqtda miqdoriy termal siklator BioRad CFX96 real vaqtda PCR tizimi BioRad CFX Opus 96 real vaqtda PCR tizimi |
Umumiy PCR eritmasi
