To'qqiz turdagi respirator viruslar
Mahsulot nomi
HWTS-RT185A-To'qqiz turdagi respirator viruslar Nuklein kislotasini aniqlash to'plami (Fluoresans PCR)
Epidemiologiya
Nafas olish yo'llari infektsiyasi har qanday jins, yosh va mintaqada paydo bo'lishi mumkin bo'lgan inson kasalliklarining eng keng tarqalgan turi bo'lib, dunyoda kasallanish va o'limning eng muhim sabablaridan biridir.[1]. Klinik jihatdan keng tarqalgan nafas yo'llarining patogenlari orasida gripp A virusi (IFV A), gripp B virusi (IFV B), yangi koronavirus (SARS-CoV-2), respirator sinsitial virus, adenovirus, inson metapnevmovirusi, rinovirus, parainfluenza virusi (I/II/III) va mikoplazma va boshqalar kiradi.[2,3]. Nafas olish yo'llari infektsiyasining klinik belgilari va belgilari nisbatan o'xshash, ammo turli patogenlar keltirib chiqaradigan infektsiya turli xil davolash usullari, davolash ta'siri va kasallikning kechishiga ega.[4,5]. Hozirgi vaqtda nafas yo'llarining patogenlarini laboratoriya aniqlashning asosiy usullari quyidagilardan iborat: virusni ajratish, antigenlarni aniqlash va nuklein kislotalarni aniqlash. Ushbu to'plam respirator virusli infektsiyani tashxislashda yordam berish uchun boshqa klinik va laboratoriya natijalari bilan birgalikda respirator infektsiyaning belgilari va alomatlari bo'lgan shaxslarda o'ziga xos virusli nuklein kislotalarni aniqlaydi va aniqlaydi.
Kanal
| FAM | MP nuklein kislotasi |
| ROX | Ichki nazorat |
Texnik parametrlar
| Saqlash | 2-8℃ |
| Yaroqlilik muddati | 12 oy |
| Namuna turi | Orofaringeal tampon; Nazofarengeal tampon |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5,0% |
| LoD | 200 nusxa/ml |
| O'ziga xoslik | O'zaro reaktivlik: To'plam va Boka virusi, Sitomegalovirus, Epstein-Barr virusi, Herpes simplex virusi, varikella zoster virusi, Parotit virusi, Enterovirus, qizamiq virusi, inson koronavirusi, SARS Koronavirus, MERS Koronavirus, Rotavirus, Norovirus, St. pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus ko'kyo'tal, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, As Candida glabpertus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella katara, Lactobacillus, Corynebacterium, inson genomik DNKsi. Interferentsiya testi: Musin (60 mg/ml), inson qoni (50%), benefrin (2 mg/ml), gidroksimetazolin (2 mg/ml) 2 mg/ml), 5% konservantli natriy xlorid (20 mg/ml), beklometazon (20 mg/mL), beklometazon (20 mg/mL), fluxmetaz (20 mg/ml), (20mkg/ml), triamsinolon (2mg/ml), budesonid (1mg/ml), mometazon (2mg/ml), flutikazon (2mg/ml), gistamin gidroxloridi (5mg/ml), benzokain (10%), mentol (10%), zanamivir/virm (20mg/ml), mupirosin (20mg/ml), tobramitsin (0,6mg/mL), oseltamivir (60ng/ml), ribavirin (10mg/l), Natijalar yuqoridagi konsentratsiyadagi aralashuvchi moddalar to'plamni aniqlashga hech qanday aralashuvchi reaktsiyaga ega emasligini ko'rsatdi. |
| Amaldagi asboblar | I turdagi aniqlash reagentiga taalluqli: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 ta real vaqtda PCR tizimi, SLAN-96P real vaqtda PCR tizimlari (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus real vaqtda PCR aniqlash tizimlari (FQD-96A, Hangzhou Bioer texnologiyasi), MA-6000 Real-Time Quantitative Quantitative Thermal, BioSuzhRy. CFX96 Real-Time PCR tizimi, BioRad CFX Opus 96 Real-Time PCR tizimi. II turdagi aniqlash reaktivi uchun qo'llaniladi: EudemonTM AIO800 (HWTS-EQ007) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
| Saqlash | 2-8℃ |
| Yaroqlilik muddati | 12 oy |
| Namuna turi | Orofaringeal tampon; Nazofarengeal tampon |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5,0% |
| LoD | 200 nusxa/ml |
| O'ziga xoslik | O'zaro reaktivlik: To'plam va Boka virusi, Sitomegalovirus, Epstein-Barr virusi, Herpes simplex virusi, varikella zoster virusi, Parotit virusi, Enterovirus, qizamiq virusi, inson koronavirusi, SARS Koronavirus, MERS Koronavirus, Rotavirus, Norovirus, St. pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus ko'kyo'tal, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, As Candida glabpertus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella katara, Lactobacillus, Corynebacterium, inson genomik DNKsi. Interferentsiya testi: Musin (60 mg/ml), inson qoni (50%), benefrin (2 mg/ml), gidroksimetazolin (2 mg/ml) 2 mg/ml), 5% konservantli natriy xlorid (20 mg/ml), beklometazon (20 mg/mL), beklometazon (20 mg/mL), fluxmetaz (20 mg/ml), (20mkg/ml), triamsinolon (2mg/ml), budesonid (1mg/ml), mometazon (2mg/ml), flutikazon (2mg/ml), gistamin gidroxloridi (5mg/ml), benzokain (10%), mentol (10%), zanamivir/virm (20mg/ml), mupirosin (20mg/ml), tobramitsin (0,6mg/mL), oseltamivir (60ng/ml), ribavirin (10mg/l), Natijalar yuqoridagi konsentratsiyadagi aralashuvchi moddalar to'plamni aniqlashga hech qanday aralashuvchi reaktsiyaga ega emasligini ko'rsatdi. |
| Amaldagi asboblar | I turdagi aniqlash reagentiga taalluqli: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 ta real vaqtda PCR tizimi, SLAN-96P real vaqtda PCR tizimlari (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus real vaqtda PCR aniqlash tizimlari (FQD-96A, Hangzhou Bioer texnologiyasi), MA-6000 Real-Time Quantitative Quantitative Thermal, BioSuzhRy. CFX96 Real-Time PCR tizimi, BioRad CFX Opus 96 Real-Time PCR tizimi. II turdagi aniqlash reaktivi uchun qo'llaniladi: EudemonTM AIO800 (HWTS-EQ007) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ish oqimi
Ibratli va mikro-test Virusli DNK/RNK to'plami (HWTS-3017) (u makro va mikro-test avtomatik nuklein kislotasi ekstraktori (HWTS-3006C, HWTS-3006B) bilan ishlatilishi mumkin) va makro va mikro-test Virusli DNK/RNK to'plami (HWTS-3017 soat bilan ishlatilishi mumkin)TM AIO800 (HWTS-EQ007)) tomonidan Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ekstraksiya qilingan namuna hajmi 200 mkl va tavsiya etilgan elutsiya hajmi 150 mkl.
