To'qqiz turdagi nafas olish viruslari
Mahsulot nomi
HWTS-RT185A-To'qqiz turdagi nafas olish viruslari nuklein kislotasini aniqlash to'plami (floresan PCR)
Epidemiologiya
Nafas olish yo'llari infektsiyasi inson kasalliklarining eng keng tarqalgan turi bo'lib, u har qanday jins, yosh va mintaqada paydo bo'lishi mumkin va dunyodagi kasallanish va o'limning eng muhim sabablaridan biridir.[1]. Klinik jihatdan keng tarqalgan nafas yo'llarining patogenlari orasida gripp A virusi (IFV A), gripp B virusi (IFV B), yangi koronavirus (SARS-CoV-2), respirator sinsitial virus, adenovirus, inson metapnevmovirusi, rinovirus, parainfluenza virusi (I/II/III) va mikoplazma va boshqalar kiradi.[2,3]Nafas olish yo'llari infektsiyasi keltirib chiqaradigan klinik alomatlar va belgilar nisbatan o'xshash, ammo turli patogenlar keltirib chiqaradigan infeksiya turli xil davolash usullariga, shifobaxsh ta'sirga va kasallikning kechishiga ega.[4,5]Hozirgi vaqtda nafas olish yo'llari patogenlarini laboratoriyada aniqlashning asosiy usullari quyidagilarni o'z ichiga oladi: virusni ajratish, antigenni aniqlash va nuklein kislotani aniqlash. Ushbu to'plam nafas olish yo'llari infektsiyasining belgilari va alomatlari bo'lgan shaxslarda o'ziga xos virusli nuklein kislotalarni aniqlaydi va boshqa klinik va laboratoriya natijalari bilan birgalikda nafas olish yo'llari virusli infektsiyasini tashxislashda yordam beradi.
Kanal
| FAM | MP nuklein kislotasi |
| ROX | Ichki nazorat |
Texnik parametrlar
| Saqlash | 2-8℃ |
| Yaroqlilik muddati | 12 oy |
| Namuna turi | Og'iz-halqum tampon; Nazofaringeal tampon |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 nusxa/ml |
| O'ziga xoslik | O'zaro reaktivlik: Kit va Boka virusi, Sitomegalovirus, Epstein-Barr virusi, Herpes simplex virusi, Varicella zoster virusi, Tepki virusi, Enterovirus, qizamiq virusi, inson koronavirusi, SARS koronavirusi, MERS koronavirusi, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, inson genom DNKsi o'rtasida o'zaro reaktivlik yo'q. Interferensiya testi: Musin (60 mg/ml), inson qoni (50%), benefrin (2 mg/ml), gidroksimetazolin (2 mg/ml) 2 mg/ml), 5% konservantli natriy xlorid (20 mg/ml), beklometazon (20 mg/ml), deksametazon (20 mg/ml), fluniatseton (20 mkg/ml), triamsinolon (2 mg/ml), budesonid (1 mg/ml), mometazon (2 mg/ml), flutikazon (2 mg/ml), gistamin gidroxlorid (5 mg/ml), benzokain (10%), mentol (10%), zanamivir (20 mg/ml), peramivir (1 mg/ml), mupirosin (20 mg/ml), tobramitsin (0.6 mg/ml), oseltamivir (60 ng/ml), ribavirin (10 mg/L), Natijalar shuni ko'rsatdiki, yuqoridagi konsentratsiyadagi aralashuvchi moddalar to'plamni aniqlashga hech qanday aralashuvchi reaksiyaga ega emas edi. |
| Amaldagi asboblar | I turdagi aniqlash reagentiga tegishli: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 ta real vaqt rejimidagi PZR tizimlari, SLAN-96P real vaqt rejimidagi PZR tizimlari (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus real vaqt rejimidagi PZR aniqlash tizimlari (FQD-96A, Hangzhou Bioer texnologiyasi), MA-6000 real vaqt rejimidagi miqdoriy termal sikler (Suzhou Molarray Co., Ltd.), BioRad CFX96 real vaqt rejimidagi PZR tizimi, BioRad CFX Opus 96 real vaqt rejimidagi PZR tizimi. II turdagi aniqlash reagentiga tegishli: Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tomonidan ishlab chiqarilgan EudemonTM AIO800 (HWTS-EQ007). |
| Saqlash | 2-8℃ |
| Yaroqlilik muddati | 12 oy |
| Namuna turi | Og'iz-halqum tampon; Nazofaringeal tampon |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 nusxa/ml |
| O'ziga xoslik | O'zaro reaktivlik: Kit va Boka virusi, Sitomegalovirus, Epstein-Barr virusi, Herpes simplex virusi, Varicella zoster virusi, Tepki virusi, Enterovirus, qizamiq virusi, inson koronavirusi, SARS koronavirusi, MERS koronavirusi, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, inson genom DNKsi o'rtasida o'zaro reaktivlik yo'q. Interferensiya testi: Musin (60 mg/ml), inson qoni (50%), benefrin (2 mg/ml), gidroksimetazolin (2 mg/ml) 2 mg/ml), 5% konservantli natriy xlorid (20 mg/ml), beklometazon (20 mg/ml), deksametazon (20 mg/ml), fluniatseton (20 mkg/ml), triamsinolon (2 mg/ml), budesonid (1 mg/ml), mometazon (2 mg/ml), flutikazon (2 mg/ml), gistamin gidroxlorid (5 mg/ml), benzokain (10%), mentol (10%), zanamivir (20 mg/ml), peramivir (1 mg/ml), mupirosin (20 mg/ml), tobramitsin (0.6 mg/ml), oseltamivir (60 ng/ml), ribavirin (10 mg/L), Natijalar shuni ko'rsatdiki, yuqoridagi konsentratsiyadagi aralashuvchi moddalar to'plamni aniqlashga hech qanday aralashuvchi reaksiyaga ega emas edi. |
| Amaldagi asboblar | I turdagi aniqlash reagentiga tegishli: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 ta real vaqt rejimidagi PZR tizimlari, SLAN-96P real vaqt rejimidagi PZR tizimlari (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus real vaqt rejimidagi PZR aniqlash tizimlari (FQD-96A, Hangzhou Bioer texnologiyasi), MA-6000 real vaqt rejimidagi miqdoriy termal sikler (Suzhou Molarray Co., Ltd.), BioRad CFX96 real vaqt rejimidagi PZR tizimi, BioRad CFX Opus 96 real vaqt rejimidagi PZR tizimi. II turdagi aniqlash reagentiga tegishli: Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tomonidan ishlab chiqarilgan EudemonTM AIO800 (HWTS-EQ007). |
Ish oqimi
Makro va mikro-test virusli DNK/RNK to'plami (HWTS-3017) (uni Makro va mikro-test avtomatik nuklein kislota ekstraktori (HWTS-3006C, HWTS-3006B) bilan ishlatish mumkin) va Makro va mikro-test virusli DNK/RNK to'plami (HWTS-3017-8) (uni Eudemon bilan ishlatish mumkin)TM AIO800 (HWTS-EQ007)) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tomonidan ishlab chiqarilgan.
Ekstraksiya qilingan namuna hajmi 200 mkL va tavsiya etilgan elyutatsiya hajmi 150 mkL.
