14 turdagi nafas olish patogenlari birlashtirilgan
Mahsulot nomi
HWTS-RT159B 14 turdagi nafas olish patogenlarini birlashtirilgan nuklein kislotani aniqlash to'plami (Fluoresans PCR)
Epidemiologiya
Nafas olish yo'llari infektsiyasi odamlarda eng keng tarqalgan kasallik bo'lib, u har qanday jins, yosh va mintaqada paydo bo'lishi mumkin. Bu butun dunyo bo'ylab aholi orasida kasallanish va o'limning asosiy sabablaridan biridir[1]. Umumiy nafas yo'llarining patogenlariga yangi koronavirus, A grippi virusi, B grippi virusi, respirator sinsitial virus, adenovirus, odam metapnevmovirusi, rinovirus, Parainfluenza virusi I/II/III/IV, Bokavirus, Enterovirus, Koronavirus, Mycoplasmae, pneamoniae, pnevmoniya va boshqalar kiradi. Streptococcus pneumoniae va boshqalar[2,3].
Kanal
| Yaxshi pozitsiyasi | Reaksiya eritmasining nomi | Aniqlanishi kerak bo'lgan patogenlar |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, ichki nazorat |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, ichki nazorat |
Texnik parametrlar
| Saqlash | ≤-18℃ |
| Yaroqlilik muddati | 9 oy |
| Namuna turi | Orofaringeal tampon, Nazofarengeal tampon |
| Ct | ≤38 |
| CV | <5,0% |
| LoD | 200Nusxa/ml |
| O'ziga xoslik | O'zaro reaktivlik testi natijalari shuni ko'rsatdiki, ushbu to'plam va Sitomegalovirus, Herpes simplex virusi 1, Varikella-zoster virusi, Epstein-Barr virusi, Bordetella ko'k yo'tal, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacilellalaghalar, Lactobacilellaghaxine, Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacterium, maltrophilia, Acinetobacterium, burnitobakteriyalarning zaiflashtirilgan shtammlari. cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, K. K. psittaci, Coxiella burnetii va inson genomik nuklein kislotalari. |
| Amaldagi asboblar | SLAN-96P Real-Time PCR tizimlari (Hongshi Medical Technology Co., Ltd.)Amaliy Biosystems 7500 Real-Time PCR tizimlari Amaliy Biosystems 7500 Fast Real-Time PCR tizimlari QuantStudio®5 real vaqtda PCR tizimlari LineGene 9600 Plus real vaqtda PCR aniqlash tizimlari (FQD-96A, Hangzhou Bioer texnologiyasi) MA-6000 real vaqtda miqdoriy termal tsikl (Suzhou Molarray Co., Ltd.) BioRad CFX96 real vaqtda PCR tizimi, BioRad CFX Opus 96 real vaqtda PCR tizimi |
Ish oqimi
Tavsiya etilgan ekstraksiya reagenti: Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tomonidan makro va mikro-test Virusli DNK/RNK to'plami (HWTS-3017) (u makro va mikro-test avtomatik nuklein kislota ekstraktori (HWTS-EQ010) bilan ishlatilishi mumkin). Olingan namuna hajmi 0 µL. Keyingi bosqichlar ushbu ekstraksiya reaktividan foydalanish bo'yicha ko'rsatmalarga muvofiq amalga oshirilishi kerak. Tavsiya etilgan elutsiya hajmi80µL.
