14 turdagi nafas olish patogenlari kombinatsiyasi
Mahsulot nomi
HWTS-RT159B 14 turdagi nafas olish patogenlari kombinatsiyalangan nuklein kislotasini aniqlash to'plami (floresan PCR)
Epidemiologiya
Nafas olish yo'llari infeksiyasi odamlarda eng keng tarqalgan kasallik bo'lib, u har qanday jins, yosh va mintaqada paydo bo'lishi mumkin. Bu butun dunyo bo'ylab aholining kasallanish va o'limning asosiy sabablaridan biridir.[1]Keng tarqalgan nafas olish yo'llari patogenlariga yangi koronavirus, A grippi virusi, B grippi virusi, respirator sinsitial virus, Adenovirus, odam metapnevmovirusi, rinovirus, I/II/III/IV tipdagi parainfluenza virusi, Bokavirus, Enterovirus, Koronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae va Streptococcus pneumoniae va boshqalar kiradi.[2,3].
Kanal
| Quduq pozitsiyasi | Reaksiya eritmasi nomi | Aniqlanadigan patogenlar |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, ichki nazorat |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, ichki nazorat |
Texnik parametrlar
| Saqlash | ≤-18℃ |
| Yaroqlilik muddati | 9 oy |
| Namuna turi | Orofaringeal tampon, Nazofaringeal tampon |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 nusxa/ml |
| O'ziga xoslik | O'zaro reaktivlik testi natijalari shuni ko'rsatdiki, ushbu to'plam bilan Sitomegalovirus, Herpes simplex virusi 1-turi, Varicella-zoster virusi, Epstein-Barr virusi, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, Mycobacterium tuberculosisning zaiflashtirilgan shtammlari, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii va inson genom nuklein kislotalari. |
| Amaldagi asboblar | SLAN-96P real vaqt rejimida PCR tizimlari (Hongshi Medical Technology Co., Ltd.)Amaliy Biosistemalar 7500 real vaqt rejimida PZR tizimlari Amaliy Biosistemalar 7500 Tezkor Haqiqiy Vaqtinchalik PZR Tizimlari QuantStudio®5 ta real vaqt rejimidagi PCR tizimlari LineGene 9600 Plus real vaqt rejimida PCR aniqlash tizimlari (FQD-96A, Hangzhou Bioer texnologiyasi) MA-6000 real vaqt rejimida miqdoriy issiqlik sikleri (Suzhou Molarray Co., Ltd.) BioRad CFX96 real vaqt rejimida PCR tizimi, BioRad CFX Opus 96 real vaqt rejimida PCR tizimi |
Ish oqimi
Tavsiya etilgan ekstraksiya reaktivi: Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tomonidan ishlab chiqarilgan Macro & Micro-Test Viral DNK/RNK to'plami (HWTS-3017) (Macro & Micro-Test Avtomatik Nuklein Kislota Ekstraktori (HWTS-EQ010) bilan ishlatilishi mumkin). Ekstraksiya qilingan namuna hajmi 200 µL ni tashkil qiladi. Keyingi bosqichlar ushbu ekstraksiya reaktividan foydalanish bo'yicha ko'rsatmalarga muvofiq bajarilishi kerak. Tavsiya etilgan elusiya hajmi...80 µL.
